Overcoming the challenges to synthesising iro

Scientists develop an efficient one-pot protocol for cell-free synthesis of complex iron-sulfur proteins

Tokyo Institute of Technology

image: This diagram shows the two main steps involved in the proposed synthesis protocol. Step 1 shows the chemical cascade used to create an oxygen-free environment, alongside the PURE system used to synthesise the 'immature' (apo) proteins. Step 2 shows the implementation of the SUF machinery, which adds the [4Fe–4S] cluster to the apo proteins, yielding functional and mature [4Fe–4S] proteins. view more

Credit: Reproduced from Wang and Nishikawa et al. 2023 ACS Synthetic Biology

Fe–S clusters, which are a part of Fe–S proteins, are found across all forms of life. They play a significant role as biological cofactors—helper molecules that assist these proteins in different biochemical transformations—that are involved in respiration and metabolism. These clusters are of keen research interest since they are considered to be a critical part of evolution. They serve as a link between pre-biotic chemistry (chemical processes that existed prior to the emergence of life forms) and the complex molecular and biological systems we know today. Put simply, they might be one of the primitive catalysts that led to the emergence of life on Earth. Thus, having convenient methods to synthesise Fe–S proteins will hopefully advance our understanding of young Earth biology and help us answer the ultimate question of the origin of life.

However, despite their prevalence, the synthesis of mature Fe–S proteins outside the cell has proven to be challenging. They not only require intricate cellular machinery for synthesis but also degrade easily upon contact with oxygen due to its reaction with their Fe–S clusters. Therefore, scientists had been forced to follow the complicated route of first producing and extracting an incomplete (or 'apo') protein, followed by its maturation (addition of the Fe–S cofactor) under strictly oxygen-deprived conditions. But what makes this process even harder is the presence of contaminating iron-containing proteins in the final extract.

In a recent study, a team of researchers, including Associate Professors Kosuke Fujishima and Shawn McGlynn from the Earth-Life Science Institute (ELSI), Tokyo Institute of Technology and Assistant Professor Po-Hsiang Wang from the National Central University developed a novel protocol for producing mature [4Fe-4S] proteins in which the Fe–S cluster is placed within a cube-like structure. The team devised a specialised Fe–S assembly protein system pathway that would function in an oxygen-free environment due to the presence of an oxygen-scavenging system to deliver mature Fe–S proteins.

The researchers first sought to assemble what's known as the sulfur formation (SUF) system. In bacteria, this multiprotein system contains all the necessary machinery to produce [4Fe-4S] clusters. It has a higher tolerance for oxygen when compared to the other pathways with similar functions (such as nitrogen fixation and theiron–sulfur cluster system). The research team created a recombinant SUF pathway that consists of six protein subunits with the ability to function in a cell-free environment.

In order to maintain an oxygen-free environment within the test tube, the researchers then introduced a three-enzyme cascade (a set of three enzymatic reactions that occur in a sequence) that serves as an oxygen-scavenging system. While this scavenging system removes oxygen from the environment, it also improves the efficiency of the system. It achieves this by producing reduced flavin adenine dinucleotide (FADH2), an electron-carrier which is needed for the synthesis of Fe-S cluster by SUF system.

Finally, for the synthesis of apo protein, the team adopted a specialised cell-free method that enables the in vitro production of proteins by using reconstituted cell-free protein synthesis known as PURE system. With the addition of genetic material (DNA or mRNA) and the necessary sources of energy, the PURE system essentially acts as an artificial protein factory.

Thus, the researchers combined the PURE system, the O2-scavenging enzyme cascade, and the components of the SUF pathway in a single tube to perform one-pot, cell-free synthesis of two representative [4Fe-4S] proteins: aconitase and thermophilic ferredoxin. Explaining the iterative processes experienced in getting the pieces of the puzzle to fit together, a co-first author PhD student Shota Nishikawa comments, "it was challenging to home-on to the appropriate stoichiometry and substrate/cofactor concentration. Nevertheless, we performed thorough studies to characterise the proper ratio of enzymes needed in the PURE system."

The protocol developed in this study has significant implications for the scientific community. "We have created a novel and convenient method for the synthesis of mature Fe–S proteins which no longer requires a bulky glove box," highlights Fujishima. He claims that their protocol overcomes the traditional challenges of the [4Fe–4S] cluster assembly and O2 sensitivity, which have been major obstacles in the fields of synthetic biology and anaerobic enzymology. By greatly extending the capabilities of the PURE system, the strategy proposed by the researchers could lead to the development of new biotechnologies and a better understanding of the fundamentals of protein synthesis and assembly.

With eyes on the future, the researchers note that this work opens several doors for upcoming studies. Being able to implement in vitro oxygen-free environments could help scientists replicate other types of multiprotein pathways, such as the nitrogen fixation (NIF) andiron–sulfur cluster (ISC) pathways, to synthesise other metal cofactor-bearing enzymes. This, in turn, may lead to the development of new biocatalysts and synthetic cells, with potential applications in environmental remediation, energy production, medicine, and astrobiology.

Reference

Po-Hsiang Wang1,2*, Shota Nishikawa3,4, Shawn Erin McGlynn3,5, and Kosuke Fujishima3,6*, One-Pot De Novo Synthesis of [4Fe-4S] Proteins Using a Recombinant SUF System under Aerobic Conditions, ACS Synthetic Biology, DOI: 10.1021/acssynbio.3c00155

More information

Tokyo Institute of Technology (Tokyo Tech) stands at the forefront of research and higher education as the leading university for science and technology in Japan. Tokyo Tech researchers excel in fields ranging from materials science to biology, computer science, and physics. Founded in 1881, Tokyo Tech hosts over 10,000 undergraduate and graduate students per year, who develop into scientific leaders and some of the most sought-after engineers in industry. Embodying the Japanese philosophy of "monotsukuri," meaning "technical ingenuity and innovation," the Tokyo Tech community strives to contribute to society through high-impact research.

The Earth-Life Science Institute (ELSI) is one of Japan's ambitious World Premiere International research centers, whose aim is to achieve progress in broadly inter-disciplinary scientific areas by inspiring the world's greatest minds to come to Japan and collaborate on the most challenging scientific problems. ELSI's primary aim is to address the origin and co-evolution of the Earth and life.

The World Premier International Research Center Initiative (WPI) was launched in 2007 by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) to help build globally visible research centers in Japan. These institutes promote high research standards and outstanding research environments that attract frontline researchers from around the world. These centers are highly autonomous, allowing them to revolutionise conventional modes of research operation and administration in Japan.

ACS Synthetic Biology

10.1021/acssynbio.3c00155

Experimental study

Not applicable

One-Pot De Novo Synthesis of [4Fe-4S] Proteins Using a Recombinant SUF System under Aerobic Conditions

19-Jul-2023

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